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Spectrophotometer is commonly used for measuring transmittance or reflectance of solutions, transparent or opaque solids like polished glass or gases. These can also be designed for measuring diffusivity on any of listed light ranges that normally covers around 200nm - 2500nm through use of different controls and calibrations. Within these ranges of light, calibration is required on machine using standards which vary in type depending on wavelength of photometric determination, The range offered includes Atomic Absorption Spectrophotometer, Microprocessor UV/Double Beam Spectrophotometer and Single/Double Beam Microprocessor Spectrophotometer. Here, while offering the solutions we also ensure that these are the best available choices made available in the market.

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Atomic Absorption Spectrophotometer

Atomic Absorption Spectrophotometer are available in a fully automated finish where the flame & graphite furnace is integrated into a single instrument. Further, the change over from one technique to another is carried out through simple key strokes within software. The instruments also come with motorized 8 hollow cathode lamp turret that allows automatic positioning & optimization of each hollow cathode lamp by software. These absorption spectrophotometers also come with automated spectral bandwidth and are available with choice of five slit sizes. Along with advanced graphite furnace and horizontally heated platform graphite tube, the unique design of graphite furnace also help in reducing chemical interference & memory effects through uniformly heating graphite electrodes. Here, the computer controlled heating program also allows users for selecting the best heating program for analysis. The optical temperature during atomization stage also ensures rapid heating and rapid analysis that allows extending life of graphite tube as well as enhance analytical accuracy.

Technical Specification
Flame Analysis
Sensitivity (Cu) 2.0µg/ml Absorption>0.2 Abs
Detection (Cu) Cu<0.0004µg/ml(flame)
Cd<0.4×10-12g(graphite furnace)
Cu<0.7%(Air-Acetylene Analysis)
Ba<1% (Nitrous Oxide Analysis)
Cu<2%,Cd<2%(Graphite Furnace Analysis)
Background Correction Deuterium Lamp>×40, Self Reversal> × 60
Character Concentration <0.02µg/ml1%(Cu)
<0.15mg/ml1%(Nitrous Oxide-Acetylene, Ba)
Character Value Cu<0.3×10-10g
Burner Head Titanium Alloy Burner
Nebulizer High-Efficiency Glass Nebulizer
Atomization Chamber Corrosion-resistant material
Position Adjustment Automatic changeover of flame and furnace
Automatic setting of height for flame burner
Safety Functions Burner identification
Flame sensor
Gas leak sensor
Low gas pressure sensor
Power loss protection
Gas loss flame protection

Features of Models
SGM 500F With flame
SGM 500G With graphite furnace
SGM 500FG With flame & graphite furnace

Optic System
Wavelength Range 190nm-900nm
Monochromator Czemy Turner Configuration
Spectral Bandwidth 0.1nm, 0.2nm, 0.4nm, 1.0nm, 2.0nm (5steps, Automatic Changeover)
Wavelength Accuracy - ±0.15nm
Wavelength Repeatability <0.05nm
Resolution <30% or 0.2nm ±0.02nm
Baseline Stability 0.005A/30min
Atomic Absorption Spectrometer Model (SGM-AA990 Series)

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Microprocessor UV/Double Beam Spectrophotometer

Microprocessor UV/Double Beam Spectrophotometer has a range from 190-1100 nm and come with features like wavelength scan, DNA Protein Test/Kinetic, automatic peak picking and spectrum display. Further, it also has large screen with graphic display with option of spectrum & data to be printed out by printer as well as sent to computer via USB PORT. Other than this, a set of 4 quartz & 4 glass cuvettes and software are also provided is provided. As an advanced Double Beam UV-VIS Spectrophotometer, it delivers more accuracy as well as flexibility in usage. The operations include use of two detectors for measuring sample & reference simultaneously for getting accurate measurement. Further, it has wide wavelength range that meets the usage requirements in different fields like -

Biochemical research and industry
  • Pharmaceuticals analysis and production
  • Education
  • Environment protection
  • Food industry

Basic Mode - For measuring the absorbance and transmittance
Quantitative - Coefficient Method

Standard Working:
Standard Curve
Up to 10 standard samples that are used for establishing a curve and four methods are used for fitting curve through calibration points.
  • Linear fit
  • Linear fit through zero
  • Square fit
  • Cubic fit

DNA/Protein Test
  • Concentration & DNA purity are quickly and easily calculated
  • Absorbance ratios 260 nm / 280 nm with optional subtracted absorbance at 320 nm
  • DNA concentration=62.9 x A260+36.0x A280 protein concentration+552xA260-757xA280

Wavelength Scan
  • Wavelength scan intervals of 0.1, 0.2, 0.5,1,2,5 nm
  • Low, Medium and High scan speeds are available
  • These scan speeds vary from 100 to 3600 nm/Min
  • Wavelengths scanned from high to low so that instrument waits at high WL
  • It minimizes degradation of UV sensitive samples

  • This mode is used for time course scanning/reaction rate calculations
  • Abs vs time graphs displayed on screen on real time
  • Wait time & measurement time up to 12 hours that may be entered with time intervals of 0.5,1,2,5,10,30 seconds & 1 min
  • Past-run manipulation that includes using linear regression algorithm before multiplying through entered factor

Glass Cells 4 No
Quartz Cells 4 No
Fuse 2 No
Instrument Cover 1 No
Software CD 1 No
USB Cable 1 No
Operational Manual 1 No

Technical Specifications:
Optical System Double Beam (1200 Lines / mm Grating)
Wavelength Range 190 – 1100 nm
Mode Basic / Quantitative / Wavelength Scan / DNA Protein Test / Kinetics
Scanning Speed Fast / Medium / Low
Band Width 1 nm
Wavelength Accuracy +/- 0.1 nm
Wavelength 0.1 nm
Photometric Accuracy +/- 0.3 % T
Photometric Repeatability 0.2 % T
Photometric Display Range 0-200%T, 0.3-3.0A, 0.9999C
Stability 0.001A/h @ 500 nm
Baseline Flatness +/- 0.001A
Noise +/- 0.001A
Stray Light < 0.005%T@ 220nm, 360nm
Data Output Port USB
Printer Port Parallel Port
Display Graphic LCD (320 x 240 Dots)
Lamps Deuterium Lamp & Tungsten Halogen Lamp
Detector Silicon Photodiodes
Dimension (L x W x H) 625 x 430 x 206 nm
Net Weight 26 Kg

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Single/Double Beam Microprocessor Spectrophotometer

Single/Double Beam Microprocessor Spectrophotometer ranges from 195 to 1000 nm and come with facilities of % Transmission, automatic concentration, absorbance & K factor measurement. Further, these also come with provision of automatic wavelength calibration & selection as well as sample holder with 4 position selector control for achieving 10 mm path length cuvettes. With set of 2 quartz and 4 glass cuvettes is provided, these also come with facility for interfacing with any printer (USB PORT). The system is also highly stable and accurate to operate with support provided by imported software that helps in handling stoca kein Test/Kinetic, automatic peak picking and spectrum display. These also have large screen with graphic display as well as spectrum and data to be printed out by printer and sent to computer via USB PORT.

Salient Features:
  • Large LCD Screen (128 x 64 dots)
  • Date can be restored after a sudden power cut
  • Automatic wavelength setting
  • Tungsten lamp & deuterium lamp can be turned on/off individually
  • Automatic wavelength calibration and dark current getting
  • Covers 200 to 1000 nm
  • 4 position cuvette Holder
  • USB & Parallel port for printing

Technical Specification:
Optical System Single Beam, Grating I 200 Lines / nm
Wavelength Range 200 – 1000 nm
Spectral Band Width 1 nm
Wavelength Accuracy +/- 0.5 nm
Wavelength Repeatability +/- 0.1 nm
Wavelength Setting Automatic
Photometric Accuracy +/- 0.1 % T
Photometric Repeatability 0.3 % T
Photometric Range -0.3 to 3.0 A, 0 to 200% T
Stray Light +/- 0.002 A/h @ 500nm
Stability 128 x 64 Dots LCD
Display Detector Silicon Photodiode
Standard Cell Holder 4 position 10 mm cell charger
Light Source Tungsten & Deuterium lamp
Output USB port & parallel port
Power AC 220V / 50 Hz
Net Weight 12 Kg

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